Fig. 2

SDS-PAGE analysis of partially purified Vip3 proteins from Bacillus thuringiensis isolates. 1, 4, and 7 indicate crude extract of HD-1 as positive control, BnBt, and MnD strains. 2, 5, and 8 indicate 60% ammonium sulfate precipitation of HD-1, BnBt, and MnD supernatants. 3, 6, and 9 indicate ion-exchange column chromatography