Table 1 Forward and reverse primers of Cry1Ac genes and Cry 3A promoter region

Primers for full length Cry1Ac gene

Forward

5’ ATGCATGCGTGAATTGCAGGTAAATGGTTC 3’ Sph1

Reverse

5’ CGGGATCCTTACTATTCCTCCATAAGGAGTAAT 3’ BamH1

Primers for coding region of Cry1Ac gene

1Ac Forward

5’ TTTGGGCCCATGGATAACAATCCGA 3’ Apa1

1Ac Reverse

5’ TTTGGGCCCTTCAGCCTCGAGTG 3’ Apa1

Primers for Cry3A promoter region

Pro Forward

5’ CGGGATCCGGACTATTATAATCAT 3’ BamH1

Pro Reverse

5’ TTTGGGCCCTTTTCTTCCTCCC3’ Apa1