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Fig. 1 | Egyptian Journal of Biological Pest Control

Fig. 1

From: Genetic improvement of Bacillus thuringiensis against the cotton bollworm, Earias vitella (Fab.) (Lepidoptera: Noctuidae), to improve the cotton yield in Pakistan

Fig. 1

Cloning of Cry1Ac coding region under Cry3A promoter in pHB201 shuttle vector (Bt1) and full-length Cry1Ac gene in pHSP9 shuttle vector (Bt2). a Gel electrophoresis of the PCR amplified products for Cry3A promoter 200 bp, Cry1Ac coding region 1.8 kb, and Cry1Ac 3.9 kb full-length gene. b Restriction digestion of positive clones with ApaI and EcoRI enzymes of expression cassette in shuttle vector pHB201 (Bt1) and full-length Cry1Ac gene in shuttle vector pHPS9 (Bt2) release fragments of specific sizes. c Plasmids map predict the theoretical sequence of recombinant strains Bt1 and Bt2 in SnapGene

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