Myco-metabolites as biological control agents against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae)

Egyptian Journal of Biological Pest Control

Table 3 Effect of ethyl acetate extract on egg hatchability of Tetranychus urticae

	Control	5 (mg/ml)	10 (mg/ml)	20 (mg/ml)	30 (mg/ml)	F value	LC₅₀ (mg/ml)	Slope
Aspergillus melleus
Mean (no. of eggs) ± SE	1.00^d ± 0.58	4.67^c ± 0.67	12.67^b ± 0.67	19.00^a ± 0.58	20.00^a ± 0.0	230.464***	8.81	3.458
% of unhatched eggs	0.00	18.52	58.92	90.91	95.96	–	8.81	3.458
Emericella nidulans
Mean (no. of eggs) ± SE	1.00^e ± 0.58	3.00^d ± 0.58	7.00^c ± 1.00	17.00^b ± 0.58	20.00^a ± 0.0	179.5***	11.66	3.923
% of unhatched eggs	0.00	10.10	30.30	80.81	95.96	–	11.66	3.923
Chaetomium globosum
Mean (no. of eggs) ± SE	1.00^e ± 0.58	4.33^d ± 0.88	10.00^c ± 1.53	13.00^b ± 0.58	20.00^a ± 0.0	73.309***	11.05	3.010
% of unhatched eggs	0.00	16.84	45.45	60.61	95.96	–	11.05	3.010
Aspergillus terreus
Mean(no. of eggs) ± SE	1.00^e ± 0.58	3.00^d ± 0.58	5.67^c ± 0.67	9.00^b ± 0.58	13.33^a ± 0.33	77.214***	23.17	1.960
% of unhatched eggs	0.00	10.10	23.57	40.40	62.29	–	23.17	1.960

Values are mean no. of unhatched eggs ± standard error of three replicates. df = 10. Different letters in the same row indicate a significant difference according to Duncan’s multiple range test
***Values are significant at P = 0.05 levels