In vitro study of biocontrol potential of rhizospheric Pseudomonas aeruginosa against Fusarium oxysporum f. sp. cucumerinum

Egyptian Journal of Biological Pest Control

Table 1 Antifungal, antagonistic, enzymatic, and plant growth promoting activity of isolate BA5

Test	Result
Antifungal activity	% mycelial growth inhibition (± SD)^a
Volatile compound(s)^b	31.11 ± 0.57**
24 h CFCF^c	45.83 ± 2.08*
48 h CFCF^d	54.16 ± 0.57**
Antagonistic properties	Activity
HCN production^e	+
CLP surfactant production^f	−
Siderophore production	+
Enzymatic properties
Protease	+
Cellulase	−
Amylase	−
Plant growth promotion properties
Phosphate solubilization^g	+
Zinc solubilization^h	+
Organic acid production	+
Nitrogenase activity	−
IAA production	−
Ammonia production	+
In vitro biofilm formation	+

^aEffect of volatile compound(s) and cell-free culture filtrate (CFCF) from isolate BA5 on mycelial growth inhibition of Foc. ^bMycelial growth was measured after 72 h of culture in sealed Petri dish. ^{c & d} CFCF obtained from 24- and 48-h-old culture broth of isolate BA5, respectively. * and ** significant at P ≤ 0.05 and P ≤ 0.001 level, respectively. ^eHCN hydrocyanic acid. ^fCLP cyclic lipopeptide. The test medium was supplemented with insoluble ^gtri-calcium phosphate and ^hzinc oxide, respectively. “+” indicates positive activity and “−” indicates no activity