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Table 3 Matrix and responses of CCD for carbon and nitrogen sources optimization

From: Optimizing production of a biopesticide protectant by black yeast

Run

Variables

Responses

SCM

Malt extract

Glycerol

CSL

Dry weight (g/l)

Melanin yield (mg/l)

1

2

2

− 2

2

3

90.88

2

0

0

0

0

3.95

55.93

3

0

− 1

0

0

5

29.30

4

1

0

0

0

3.4

66.35

5

2

2

− 2

− 2

3.5

77.01

6

2

− 2

2

− 2

3.2

59.77

7

0

0

0

− 1

0

0

8

− 2

2

2

2

6

54.84

9

− 2

2

− 2

− 2

3.5

35.09

10

0

0

1

0

3.4

73.87

11

− 2

− 2

2

2

2.2

61.18

12

0

0

0

0

3.95

55.93

13

− 1

0

0

0

3.5

78.34

14

2

2

2

− 2

10

39.17

15

− 2

2

− 2

2

2.5

68.0

16

2

− 2

− 2

2

4

97.14

17

0

0

0

1

3.4

82.10

18

2

− 2

2

2

4

77.56

19

0

0

0

0

3.95

55.93

20

− 2

2

2

− 2

5

17.39

21

0

1

0

0

3.1

49.90

22

− 2

− 2

− 2

− 2

1.4

56.48

23

0

0

0

0

3.95

55.93

24

2

− 2

− 2

− 2

3.3

62.67

25

2

2

2

2

3.1

76.30

26

− 2

− 2

2

− 2

2.2

37.60

27

− 2

− 2

− 2

2

3

63.22

28

0

0

0

0

3.95

55.93

29

0

0

0

0

3.95

55.93

30

0

0

0

0

3.95

55.93

31

0

0

− 1

0

6.1

71.60

  1. Maximum and minimum yields were displayed as italics