Table 1 PCR conditions and primer sequence of ITS and COI genes of B. bassiana
Gene | Product size (bp) | Annealing (°C) | Direction | Sequence (5′-3′) |
|---|---|---|---|---|
ITS | 593 | 50 | Sense | GGAAGTAAAAGTCGTAACAA |
Antisense | TCCTCGCTTATTGATATGA | |||
COI | 437 | 50 | Sense | TGCCTTTAATGGTAGGAGGTC |
Antisense | AGGATCTCCACCACCAGCTA |