Efficacy of two species of entomopathogenic fungi against the stored-grain pest, Sitophilus granarius L. (Curculionidae: Coleoptera), via oral ingestion

Egyptian Journal of Biological Pest Control

Table 1 Isolation of ingested fungus conidia (or mycelium developing from them) of Metarhizium anisopliae from infected adults of granary weevil, Sitophilus granarius, after feeding them on mixtures of M. anisopliae conidia + wheat flour for 24 h then incubated after surface treatment with Merpan® on healthy rice grains at 20 °C

Time (days) for isolation the fungus ^c	Mixture ratios of M. anisopliae conidia and wheat flour (w/w)^a
	T1 (0.2:2.0 g/g)		T2 (0.1:2.0 g/g)		T3 (0.05:2.0 g/g)		T4 (0:2.0 g/g)
	Treated^b	Untreated^b	Treated^b	Untreated^b	Treated^b	Untreated^b	Treated^b	Untreated^b
1 day	−^d	−^d	−^d	−^d	−^d	−^d	−^d	−^d
2 days	−	+	−	+	−	+	−	−
3 days	+	+	+	+	+	+	−	−
7 days	+	+ (first death)	+	+	+	+	−	−
8 days	+		+	+ (first death)	+	+	−	−
9 days	+		+		+	+ (first death)	−	−
12 days	+ (first death)		+		+		−	−
13 days			+ (first death)		+ (first death)		−	−

^aFour replicates were used per treatment and each replicate contains 20 adults of S. granarius
^bTreated or untreated adults of S. oryzae with the contact fungicide Merpan®
^cTime in days from treatment with Merpan® to starting of fungus isolation on a selective culture medium
^d+ means positive isolation of the fungus on a selective culture medium (Sabouraud Dextrose Agar + Chloramphenicol 250 mg/L); − means negative isolation of the fungus