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Table 4 Effect of Macrophomina phaseolina, soil amendment, and Trichoderma spp. on physiological attributes in Vigna unguiculata

From: Anti-mycotic potential of Trichoderma spp. and leaf biomass of Azadirachta indica against the charcoal rot pathogen, Macrophomina phaseolina (Tassi) Goid in cowpea

Treatments

Total chlorophyll content (mg/g)

CAT U/min per mg of protein

POX U/min per mg of protein

PPO U/min per mg of protein

PAL U/min per mg of protein

T1: negative control (without any inoculation or amendment)

0.52e

3.54e

1.43f

0.017cd

0.11c

T2: positive control [(inoculated with Macrophomina phaseolina (MP) only)]

0.19f

5.50a

3.05a

0.04a

0.18a

T3: MP + 1% A. indica

0.53e

(− 165%)

4.51b

(18%)

2.41c

(21%)

0.032b

(20%)

0.17a

(29%)

T4: MP + 2% A. indica

0.56d

(− 180%)

4.12c

(25%)

2.44c

(21%)

0.031b

(23%)

0.15d

(29%)

T5: MP + 3% A. indica

0.59d

(− 195%)

4.12c

(25%)

2.18d

(29%)

0.031b

(23%)

0.14b

(24%)

T6: MP + T. harzianum

0.66cd

(− 225%)

3.78d

(31%)

1.80e

(41%)

0.022c

(45%)

0.11c

(39%)

T7: MP + T. harzianum + 1% A. indica

0.73b

(− 265%)

3.73d

(33%)

1.57f

(49%)

0.025bc

(38%)

0.11c

(45%)

T8: MP + T. harzianum + 2% A. indica

0.79ab

(− 295%)

3.62e

(35%)

1.44f

(53%)

0.023c

(43%)

0.11c

(45%)

T9: MP + T. harzianum + 3% A. indica

0.84a

(− 320)

3.61e

(35%)

1.41f

(54%)

0.022c

(45%)

0.09c

(45%)

T10: MP + T. viride

0.59d

(− 193%)

3.98c

(28%)

2.58b

(15%)

0.028bc

(30%)

0.14b

(32%)

T11: MP + T. viride + 1% A. indica

0.64c

(−220%)

3.81d

(31%)

2.41c

(21%)

0.029 bc

(28%)

0.16 ab

(29%)

T12: MP + T. viride + 2% A. indica

0.67bc

(− 235%)

3.71d

(33%)

2.25d

(26%)

0.029b

(28%)

0.15ab

(26%)

T13: MP + T. viride + 3% A. indica

0.71b

(− 255%)

3.67de

(33%)

2.16d

(29%)

0.029b

(27%)

0.14b

(26%)

  1. Values with different letters in column show significant difference (P ≤ 0.05) as determined by LSD test. Values in parenthesis show increase/decrease in treatment with respect to positive control