Table 2 Experimental replicates, sets of bioassay arenas, and total sample sizes of arenas per tested entomopathogenic nematode and applied concentration in soil-based laboratory bioassays (concentration = 100, 200, 300, 400 and/or 1000 infective juveniles (IJs) per grub equalling 4, 8, 12, 16 and 40 IJs per gram soil, 2.4, 4.8, 7.1 and 9.5 IJs per cm3 soil and 14, 28, 42, 57, and 140 IJs per cm2 soil surface). An experiment consisted of at least two sets of 10 bioassay arenas each, and each arena containing one Anomala graueri grub
From: Management of white grubs (Coleoptera: Scarabeidae) with entomopathogenic nematodes in Rwanda
Treatment | Experiments/(sets of 10 arenas across experiments)/sample size of arenas across experiments | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Concentration (IJ per larva) | |||||||||||||||||
| Â | 100 | 200 | 300 | 400 | 1000 | Total | ||||||||||||
H. bacteriophora RW14-N-C4a | 3 | (9) | 90 | 2 | (6) | 60 | 2 | (6 | 60 | 3 | (9 | 90 | 2 | (6 | 60 | 12 | (36) | 360 |
H. bacteriophora H06 | 5 | (15) | 150 | 5 | (15) | 150 | 4 | (12) | 120 | 5 | (15) | 150 | 2 | (6) | 60 | 21 | (63) | 630 |
S. carpocapsae All | 3 | (9) | 90 | 2 | (6 | 60 | 1 | (3) | 30 | 1 | (3) | 30 | 2 | (6) | 60 | 9 | (27) | 270 |
S. carpocapsae RW14-G-R3a-2 | 3 | (9) | 90 | 3 | (9) | 90 | 2 | (6) | 60 | 3 | (9) | 90 | 4 | (12) | 120 | 15 | (45) | 450 |
Steinernema sp. RW14-M-C2a-3 | 3 | (9) | 90 | 2 | (6) | 60 | 2 | (6) | 60 | 3 | (9) | 90 | 2 | (6) | 60 | 12 | (36) | 360 |
Steinernema sp. RW14-M-C2b-1 | 3 | (9) | 90 | 3 | (9) | 90 | 2 | (6) | 60 | 3 | (9) | 90 | 4 | (12) | 120 | 15 | (45) | 450 |
S. longicaudum X7 | 3 | (9) | 90 | 3 | (9) | 90 | 2 | (6) | 60 | 3 | (9) | 90 | 2 | (6) | 60 | 13 | (39) | 390 |
Untreated control | Similar numbers for each experiment | |||||||||||||||||